7FEA
PY14 in complex with Col-D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-08-30 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.943, 77.741, 98.117 |
| Unit cell angles | 70.76, 81.78, 65.00 |
Refinement procedure
| Resolution | 27.160 - 1.400 |
| R-factor | 0.1464 |
| Rwork | 0.146 |
| R-free | 0.16270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n44 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.056 | 0.553 |
| Rpim | 0.040 | 0.404 |
| Number of reflections | 314912 | 28574 |
| <I/σ(I)> | 16.3 | 2 |
| Completeness [%] | 94.8 | 86 |
| Redundancy | 2.9 | 2.5 |
| CC(1/2) | 0.914 | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 283 | 0.2 M sodium malonate, 20% (w/v) polyethylene glycol 3350, 0.1 M Bis-tris propane pH 6.5 |
