7FE3

Crystal structure of GH65 alpha-1,2-glucosidase from Flavobacterium johnsoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-27
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	122.830, 193.970, 111.735
Unit cell angles	90.00, 116.60, 90.00

Refinement procedure

Resolution	48.540 - 1.540
Rwork	0.171
R-free	0.19180
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.501
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.620
High resolution limit [Å]	1.540	1.540
Rmerge	0.050	0.916
Rmeas	0.054	0.992
Rpim	0.021	0.379
Number of reflections	342453	49838
<I/σ(I)>	19.9	2.3
Completeness [%]	99.7
Redundancy	6.8
CC(1/2)	1.000	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	300 mM ammonium citrate, pH 7.0-8.0, 10 mM TCEP, 12% PEG 3350