7FDO
Crystal structure of transcription factor CPC in complex with EGL3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 86.054, 50.358, 81.657 |
Unit cell angles | 90.00, 97.23, 90.00 |
Refinement procedure
Resolution | 28.701 - 1.752 |
R-factor | 0.1993 |
Rwork | 0.198 |
R-free | 0.22320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rru |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (7.0.076) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
Rmerge | 0.024 | 0.018 | 0.313 |
Rmeas | 0.026 | 0.020 | 0.348 |
Rpim | 0.010 | 0.008 | 0.147 |
Total number of observations | 209244 | ||
Number of reflections | 34121 | 3247 | 3438 |
<I/σ(I)> | 20.9 | ||
Completeness [%] | 97.5 | 90.2 | 98.9 |
Redundancy | 6.1 | 6.2 | 5.2 |
CC(1/2) | 0.999 | 0.275 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris-HCl pH 6.5, 20% PEG3350, 0.2 M ammonium sulfate |