7FD3

IL-1RAPL2 TIR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-27
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	51.141, 51.141, 184.779
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.290 - 2.990
R-factor	0.2424
Rwork	0.240
R-free	0.28250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3g
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.290	3.150
High resolution limit [Å]	2.990	2.990
Rmerge	0.155	1.716
Number of reflections	5452	515
<I/σ(I)>	19.6
Completeness [%]	99.8
Redundancy	24

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	8% v/v tacsimate pH5.0, 12% w/v PEG3350