7FD1
7-FE FERREDOXIN FROM AZOTOBACTER VINELANDII AT PH 8.5, 100 K, 1.35 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1998-10 |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 54.460, 54.460, 92.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 1.300 |
R-factor | 0.158 |
Structure solution method | OTHER |
Starting model (for MR) | 6fd1 |
RMSD bond length | 0.011 |
RMSD bond angle | 2.210 * |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | TRIVIAL (CASE) |
Refinement software | SHELXL (97) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.400 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.082 * | 0.340 * |
Total number of observations | 122037 * | |
Number of reflections | 29311 | |
<I/σ(I)> | 17.9 | 0.82 |
Completeness [%] | 82.8 | 51.5 |
Redundancy | 3.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.4 * | 4 * | PROTEIN CRYSTALLIZED FROM 60% SAT. AMMONIUM SULFATE, 0.4M TRIS-HCL, PH 7.8 THE CRYSTALS WERE SOAKED IN 0.15M TRIS/HCL PH 8.5 BEFORE DATA COLLECTION. STARTING MODEL IN THE REFINEMENT WAS PDB ENTRY 6FD1. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 7-10 (mg/ml) | |
2 | 1 | 1 | potassium phosphate | 0.5 (M) | |
3 | 1 | 1 | ammonium sulfate | 1.2 (M) | |
4 | 1 | 2 | ammonium sulfate | 3.5 (M) |