7FCW
X-ray structure of H2O-solvent lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2019-06-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.216, 79.216, 37.823 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.430 - 1.430 |
R-factor | 0.1687 |
Rwork | 0.167 |
R-free | 0.18580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7fcu |
RMSD bond length | 0.006 |
RMSD bond angle | 1.017 |
Data reduction software | XDS (Mar 15,2019) |
Data scaling software | XDS (Mar 15,2019) |
Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.820 | 1.450 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.063 | 0.676 |
Rmeas | 0.065 | 0.701 |
Rpim | 0.017 | 0.182 |
Number of reflections | 22878 | 1136 |
<I/σ(I)> | 26.1 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 14.7 | 14.6 |
CC(1/2) | 0.999 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 4.5 | 293 | 15-25mg/mL HEWL, 50mM sodium acetate (pH4.5), 0.1g/mL NiCl2 |