7FCR
Crystal structure of the N-terminal domain of mutants of Human Apolipoprotein-E (ApoE)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-02-15 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.642, 53.207, 85.982 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.820 - 1.400 |
R-factor | 0.1717 |
Rwork | 0.171 |
R-free | 0.19370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gs9 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.240 | 45.240 | 1.420 |
High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
Rmerge | 0.037 | 0.038 | 0.940 |
Rmeas | 0.040 | 0.042 | 1.012 |
Rpim | 0.015 | 0.018 | 0.373 |
Total number of observations | 267298 | 1500 | 12971 |
Number of reflections | 37333 | 276 | 1803 |
<I/σ(I)> | 26.1 | 55.9 | 2.2 |
Completeness [%] | 99.6 | 97.9 | 99.7 |
Redundancy | 7.2 | 5.4 | 7.2 |
CC(1/2) | 0.999 | 0.997 | 0.709 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.15 | 294 | 100 mM sodium cacodylate, 28% PEG 400 |