7FCL

Zebrafish SIGIRR TIR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9796
Spacegroup name	C 2 2 2
Unit cell lengths	83.996, 145.364, 75.663
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.560 - 3.040
R-factor	0.2408
Rwork	0.239
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fcj
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.090
High resolution limit [Å]	3.040	3.040
Rmerge	0.091	0.689
Number of reflections	9184	455
<I/σ(I)>	11.1
Completeness [%]	99.8
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.05 M Ammonium sulfate, 0.05 M BIS-TRIS pH6.9, 34% v/v Pentaerythritol ethoxylate (15/4 EO/OH), 3% v/v Glycerol