7FCJ

Zebrafish SIGIRR TIR domain mutant - C299S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-30
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	31.744, 61.612, 69.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.110 - 1.880
R-factor	0.1997
Rwork	0.198
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fcc
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.880	1.880
Rmerge	0.130	0.537
Number of reflections	10663	631
<I/σ(I)>	13
Completeness [%]	91.8
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M CHES pH 9.5, 20% w/v PEG8000