7FCH
IL-18Rbeta TIR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-05 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.760, 161.074, 52.164 |
| Unit cell angles | 90.00, 112.44, 90.00 |
Refinement procedure
| Resolution | 48.214 - 1.883 |
| R-factor | 0.2 |
| Rwork | 0.186 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7fcc |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.083 | 0.497 |
| Number of reflections | 46801 | 4060 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 95.6 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Ammonium tartrate dibasic, pH 6.25, 20% w/v PEG3350, 3% v/v (+/-)-2-Methyl-2,4-pentanediol |
