7FCB
SptF 9 residues truncated mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-27 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.210, 52.316, 57.784 |
| Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
| Resolution | 45.100 - 1.400 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.19550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7eyr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.949 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.036 | 0.374 |
| Number of reflections | 52223 | 2577 |
| <I/σ(I)> | 12.4 | 2.2 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.999 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M sodium formate (pH 7.0), 20% w/v PEG 3350 |
