7FC5

Crystal structure of SARS-CoV-2 RBD and horse ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	I 41 2 2
Unit cell lengths	196.173, 196.173, 144.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.735 - 2.894
R-factor	0.2302
Rwork	0.229
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.735	2.990
High resolution limit [Å]	2.890	2.890
Number of reflections	30247	2521
<I/σ(I)>	10.11
Completeness [%]	95.5
Redundancy	16.9
CC(1/2)	0.986	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2M Sodium formate, 20% w/v polyethylene glycol 3350