7FC3

structure of NL63 receptor-binding domain complexed with horse ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-19
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.9798
Spacegroup name	P 61 2 2
Unit cell lengths	112.905, 112.905, 328.924
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.230 - 3.190
R-factor	0.235
Rwork	0.232
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kbh
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.230	3.300
High resolution limit [Å]	3.190	3.190
Number of reflections	21422	2019
<I/σ(I)>	15.6
Completeness [%]	98.8
Redundancy	27.4
CC(1/2)	0.978	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	1.8M Ammonium sulfate, 0.1M BIS-TRIS pH6.5, 2% v/v polyethylene glycol monomethylether 550