7FC2
Crystal Structure of GPX6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 52.390, 108.922, 120.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.030 - 2.000 |
R-factor | 0.1902 |
Rwork | 0.189 |
R-free | 0.20750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r37 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.034 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.134 | 0.646 |
Rpim | 0.048 | 0.211 |
Number of reflections | 44094 | 2311 |
<I/σ(I)> | 20.17 | 5.94 |
Completeness [%] | 98.9 | 98.84 |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.6 M ammonium sulfate, 0.1 M sodium citrate |