7FBW
Acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 62.211, 105.992, 118.077 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.710 - 1.900 |
Rwork | 0.186 |
R-free | 0.21010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cc0 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.822 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.710 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.484 |
Number of reflections | 30651 | 1986 |
<I/σ(I)> | 9.1 | 1.9 |
Completeness [%] | 98.7 | |
Redundancy | 3.5 | |
CC(1/2) | 0.994 | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M CHES-NaOH pH 9.5, 10% PEG3350, 0.022 M strontium chloride |