7FBH
geranyl pyrophosphate C6-methyltransferase BezA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2019-05-14 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 85.666, 118.670, 196.111 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.340 - 2.100 |
R-factor | 0.2029 |
Rwork | 0.201 |
R-free | 0.23640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.021 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.340 | 2.100 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.145 | 1.238 |
Rmeas | 0.148 | 1.295 |
Rpim | 0.030 | 0.363 |
Number of reflections | 57399 | 3899 |
<I/σ(I)> | 21.1 | 2.5 |
Completeness [%] | 98.0 | 87.1 |
Redundancy | 24.2 | 12.9 |
CC(1/2) | 0.997 | 0.719 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES (pH 7.5), 25% PEG3350, 0.2 M CH3COONH4. 0.01 M spermine tetrahydrochloride |