7FBH

geranyl pyrophosphate C6-methyltransferase BezA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-05-14
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	I 2 2 2
Unit cell lengths	85.666, 118.670, 196.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.340 - 2.100
R-factor	0.2029
Rwork	0.201
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.340	2.100
High resolution limit [Å]	2.100	2.100
Rmerge	0.145	1.238
Rmeas	0.148	1.295
Rpim	0.030	0.363
Number of reflections	57399	3899
<I/σ(I)>	21.1	2.5
Completeness [%]	98.0	87.1
Redundancy	24.2	12.9
CC(1/2)	0.997	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES (pH 7.5), 25% PEG3350, 0.2 M CH3COONH4. 0.01 M spermine tetrahydrochloride