7FB5
Crystal structure of FAM134B/GABARAP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.250, 73.250, 80.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.950 - 2.840 |
| R-factor | 0.1892 |
| Rwork | 0.186 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yir |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.18.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.840 | 7.730 | 2.850 |
| Rmerge | 0.109 | 0.036 | 1.174 |
| Rmeas | 0.113 | 0.037 | 1.213 |
| Rpim | 0.028 | 0.009 | 0.301 |
| Number of reflections | 6205 | 355 | 310 |
| <I/σ(I)> | 3.3 | ||
| Completeness [%] | 99.9 | 98.3 | 99.7 |
| Redundancy | 15.6 | 13.7 | 15.9 |
| CC(1/2) | 1.000 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | containing 0.1 M bis-tris propane pH 8.0, 2.2 M DL-Malic acid |
