7FAW
Structure of LW domain from Yeast
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.014, 91.568, 118.068 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.179 - 2.438 |
R-factor | 0.2054 |
Rwork | 0.202 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | model built by rosetta |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 118.070 | 2.500 |
High resolution limit [Å] | 2.438 | 2.440 |
Rmerge | 0.104 | 0.621 |
Number of reflections | 24984 | 996 |
<I/σ(I)> | 20.2 | 4.8 |
Completeness [%] | 100.0 | |
Redundancy | 14 | |
CC(1/2) | 0.999 | 0.958 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Tris, pH 8.5, 25% PEG 3350 |