7FAN
Co-crystal Structure of Toxoplasma gondii Prolyl tRNA Synthetase (TgPRS) in complex with T35 and L-pro
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.522, 74.296, 75.073 |
Unit cell angles | 90.00, 111.24, 90.00 |
Refinement procedure
Resolution | 36.533 - 1.769 |
R-factor | 0.1767 |
Rwork | 0.175 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6aa0 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.15rc2_3428) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.650 | 1.820 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 57267 | 4208 |
<I/σ(I)> | 9.2 | |
Completeness [%] | 100.0 | |
Redundancy | 6.5 | |
CC(1/2) | 0.990 | 0.512 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Morpheus H2 |