7FAH

Immune complex of head region of CA09 HA and neutralizing antibody 12H5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	123.15
Detector technology	CCD
Collection date	2016-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	107.740, 51.122, 168.248
Unit cell angles	90.00, 106.90, 90.00

Refinement procedure

Resolution	38.606 - 3.151
R-factor	0.2674
Rwork	0.266
R-free	0.28950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lzg 1c1e
RMSD bond length	0.003
RMSD bond angle	0.676
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.150	3.150
Rpim	0.077
Number of reflections	30642	2709
<I/σ(I)>	19.2
Completeness [%]	99.8
Redundancy	11.8
CC(1/2)	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M MES pH 6.5, 13% (w/v) PEG 2000