7FAD
Crystal structure of Xenopus GCP2-N terminal domain and Mzt2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2020-01-18 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.99 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 82.878, 125.099, 204.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.957 - 3.204 |
R-factor | 0.2094 |
Rwork | 0.206 |
R-free | 0.23740 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.598 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.290 |
High resolution limit [Å] | 3.170 | 3.170 |
Rmeas | 0.134 | |
Number of reflections | 18101 | 1608 |
<I/σ(I)> | 25.33 | |
Completeness [%] | 98.5 | 89.7 |
Redundancy | 6.5 | |
CC(1/2) | 0.909 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Sodium formate pH 7, 12% PEG 3350 |