7FAC
Crystal Structure of C-terminus of the non-structural protein 2 from SARS coronavirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-20 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 65 |
Unit cell lengths | 112.834, 112.834, 91.084 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.930 - 2.710 |
R-factor | 0.2002 |
Rwork | 0.197 |
R-free | 0.25700 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
Rmerge | 0.131 | 0.072 | 0.658 |
Total number of observations | 221024 | ||
Number of reflections | 18044 | 941 | 897 |
<I/σ(I)> | 8.7 | ||
Completeness [%] | 99.5 | 98.6 | 98.5 |
Redundancy | 12.2 | 10.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Bis-Tris (pH 6.5), 0.2M NaCl, 20% (w/v) PEG 5000 monomethy ether with IPTG |