7F9X
Structure of the second OTU domain of LotA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 77.150, 94.120, 83.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.790 - 1.970 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22760 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.018 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.790 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.094 | 0.517 |
| Rpim | 0.042 | 0.250 |
| Number of reflections | 21904 | 1502 |
| <I/σ(I)> | 11.1 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.9 | 6.1 |
| CC(1/2) | 0.997 | 0.833 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 39% (w/v) PEG200, 0.1 M NaOAc pH4.5 and 100 mM NaCl |
