7F9I

The apo-form structure of EnrR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-25
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97928
Spacegroup name	P 21 21 2
Unit cell lengths	56.283, 72.379, 34.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.140 - 2.500
R-factor	0.2449
Rwork	0.242
R-free	0.28940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7f9h
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.590
High resolution limit [Å]	2.480	5.380	2.500
Rmerge	0.143	0.116	0.382
Rmeas	0.153	0.123	0.428
Rpim	0.054	0.040	0.187
Number of reflections	5129	586	483
<I/σ(I)>	7
Completeness [%]	97.2	98.8	93.2
Redundancy	6.1	8.2	4.2
CC(1/2)		0.993	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	PEG3350 and sodium formate