7F9D
Homo sapiens Prolyl-tRNA Synthetase (HsPRS) in Complex with L-proline and compound L96
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-03 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.490, 106.130, 145.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.125 - 2.497 |
R-factor | 0.1907 |
Rwork | 0.187 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vad |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15rc1_3423) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.125 | 2.650 |
High resolution limit [Å] | 2.497 | 2.497 |
Number of reflections | 73020 | 6158 |
<I/σ(I)> | 9.23 | |
Completeness [%] | 99.7 | 98.5 |
Redundancy | 13.2 | |
CC(1/2) | 0.990 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, 0.5 M CaCl2, 20% PEG 3350 |