7F90
Crystal structure of SARS auxiliary protein in complex with human nuclear protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.332, 86.794, 47.905 |
| Unit cell angles | 90.00, 92.09, 90.00 |
Refinement procedure
| Resolution | 47.870 - 2.390 |
| R-factor | 0.2215 |
| Rwork | 0.220 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4owr |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.874 | 2.540 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.131 | 0.624 |
| Number of reflections | 60024 | 9702 |
| <I/σ(I)> | 7.62 | 2.01 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 3.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | 0.1m Bis-tris ph5.5,45%PEG4000 |
