7F8Z

RNA kink-turn motif with 2-aminopurine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-19
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	65.962, 87.726, 124.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.040 - 3.000
R-factor	0.1809
Rwork	0.176
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c40
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.040	100.000	3.100
High resolution limit [Å]	3.000	5.000	3.000
Rmerge	0.099	0.050	0.396
Rmeas	0.109	0.055	0.436
Number of reflections	14856	3307	1348
<I/σ(I)>	14.66	23.87	5.23
Completeness [%]	98.7	97.3	99.2
Redundancy	5.796	5.487	5.852
CC(1/2)	0.997	0.998	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MPD, spermine tetrahydrochloride, potassium chloride