7F8T

Re-refinement of the 2XRY X-ray structure of archaeal class II CPD photolyase from Methanosarcina mazei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Detector technology	CCD
Collection date	2008-02-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.88567
Spacegroup name	P 43 21 2
Unit cell lengths	69.770, 69.770, 243.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.940 - 1.500
R-factor	0.1704
Rwork	0.170
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xry
RMSD bond length	0.018
RMSD bond angle	1.488
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.5 M LITHIUM SULFATE, 7.5% (W/V) PEG 8000