7F8L

Crystal structure of Bat coronavirus RaTG13 ORF8 accessory protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	37.473, 49.454, 111.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.950 - 1.762
R-factor	0.1859
Rwork	0.181
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jtl
RMSD bond length	0.007
RMSD bond angle	0.868
Data scaling software	HKL-3000
Refinement software	PHENIX (1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.760	1.760
Rmeas	0.114
Number of reflections	21199	1327
<I/σ(I)>	17.2
Completeness [%]	95.9
Redundancy	8.7
CC(1/2)		0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	160 mM Calcium chloride, 80 mM Sodium HEPES pH 7.5, 23% (v/v) PEG400