7F8K
Room temperature structure of bacterial copper amine oxidase determined by serial femtosecond crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL2 |
Synchrotron site | SACLA |
Beamline | BL2 |
Temperature [K] | 300 |
Detector technology | CCD |
Collection date | 2018-06-02 |
Detector | MPCCD |
Wavelength(s) | 1.771 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 158.750, 64.740, 93.550 |
Unit cell angles | 90.00, 112.33, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.200 |
R-factor | 0.1555 |
Rwork | 0.154 |
R-free | 0.17880 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 0.889 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASES |
Refinement software | PHENIX (dev_3958) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.278 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 87284 | 8717 |
<I/σ(I)> | 7.76 | |
Completeness [%] | 100.0 | |
Redundancy | 147.3 | |
CC(1/2) | 0.986 | 0.618 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.4 | 289 | 1.05 M potassium-sodium tartrate in 25 mM HEPES buffer |