7F8F
Roadblock from Odinarchaeota LCB_4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-10 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 52.072, 59.245, 59.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.300 - 1.830 |
Rwork | 0.193 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l7h |
RMSD bond length | 0.002 |
RMSD bond angle | 0.448 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | ACORN |
Refinement software | PHENIX (v1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.100 | 1.870 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.046 | 0.524 |
Rmeas | 0.050 | 0.566 |
Rpim | 0.019 | 0.211 |
Number of reflections | 8418 | 813 |
<I/σ(I)> | 18.5 | 2.6 |
Completeness [%] | 99.8 | |
Redundancy | 7.1 | |
CC(1/2) | 0.889 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 200 mM MgSO4 20% PEG 4000 10% glycerol |