7F8B
Crystal structure of rRNA methyltransferase Erm38 in complex with SAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 78.018, 78.018, 101.536 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.470 - 2.248 |
R-factor | 0.2129 |
Rwork | 0.211 |
R-free | 0.24780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7f8a |
RMSD bond length | 0.008 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.470 | 48.470 | 2.320 |
High resolution limit [Å] | 2.248 | 8.990 | 2.250 |
Rmerge | 0.079 | 0.023 | 0.800 |
Rmeas | 0.080 | 0.024 | 0.818 |
Rpim | 0.016 | 0.005 | 0.167 |
Total number of observations | 6478 | 31343 | |
Number of reflections | 15503 | 308 | 1344 |
<I/σ(I)> | 31.3 | 95.3 | 4.4 |
Completeness [%] | 99.8 | 99.6 | 97.5 |
Redundancy | 25.9 | 21 | 23.3 |
CC(1/2) | 1.000 | 1.000 | 0.937 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.0 M succinic acid pH 7, 5% glycerol |