7F8A
Crystal structure of rRNA methyltransferase Erm38
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 77.663, 77.663, 101.116 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.260 - 1.900 |
| R-factor | 0.2101 |
| Rwork | 0.209 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ftf 3fuv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.260 | 48.250 | 2.030 |
| High resolution limit [Å] | 1.900 | 8.830 | 1.970 |
| Rmerge | 0.066 | 0.023 | 1.570 |
| Rmeas | 0.067 | 0.024 | 1.603 |
| Rpim | 0.013 | 0.005 | 0.315 |
| Total number of observations | 6115 | 38643 | |
| Number of reflections | 27116 | 317 | 1559 |
| <I/σ(I)> | 30.6 | 89.2 | 2.1 |
| Completeness [%] | 99.7 | 99.6 | 95.9 |
| Redundancy | 25.9 | 19.3 | 24.8 |
| CC(1/2) | 1.000 | 1.000 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.0 M succinic acid pH 7, 5% glycerol |
