7F88
Crystal structure of GH19 chitinase lacking the third loop structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2015-12-16 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.260, 103.647, 45.418 |
Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
Resolution | 45.240 - 1.600 |
R-factor | 0.1474 |
Rwork | 0.146 |
R-free | 0.17510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wh1 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.153 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.068 | 0.427 |
Rpim | 0.029 | 0.191 |
Number of reflections | 70086 | 3458 |
<I/σ(I)> | 20.7 | 2.5 |
Completeness [%] | 99.4 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium citrate dibasic, 20 % w/v polyethylene glycol 3350, pH 5.1 |