7F86
Crystal structure of Phycoerythrin from Halomicronema Sp. R31DM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2019-10-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 108.590, 108.630, 116.930 |
| Unit cell angles | 78.37, 82.53, 62.11 |
Refinement procedure
| Resolution | 42.170 - 2.210 |
| R-factor | 0.1654 |
| Rwork | 0.163 |
| R-free | 0.21010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nb4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.575 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.170 | 2.210 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 225119 | 11005 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 97.3 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.997 | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M Potassium sodium tartrate, 20% (W/V) PEG 3350 |
