7F86
Crystal structure of Phycoerythrin from Halomicronema Sp. R31DM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2019-10-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 108.590, 108.630, 116.930 |
Unit cell angles | 78.37, 82.53, 62.11 |
Refinement procedure
Resolution | 42.170 - 2.210 |
R-factor | 0.1654 |
Rwork | 0.163 |
R-free | 0.21010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nb4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.575 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.170 | 2.210 |
High resolution limit [Å] | 2.210 | 2.210 |
Number of reflections | 225119 | 11005 |
<I/σ(I)> | 15.6 | |
Completeness [%] | 97.3 | |
Redundancy | 3.9 | |
CC(1/2) | 0.997 | 0.974 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M Potassium sodium tartrate, 20% (W/V) PEG 3350 |