7F84

Crystal structure of CRISPR-associated Cas2c of Leptospira interrogans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-04-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 4 2 2
Unit cell lengths	103.160, 103.160, 97.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.940 - 2.600
R-factor	0.2117
Rwork	0.209
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qr0
RMSD bond length	0.013
RMSD bond angle	1.869
Data reduction software	MOSFLM (7.3.0)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.940	72.940	2.720
High resolution limit [Å]	2.600	9.010	2.600
Rmerge	0.162	0.076	0.516
Rmeas	0.167	0.080	0.532
Rpim	0.040	0.022	0.129
Total number of observations	140737	3389	17109
Number of reflections	8390	246	1011
<I/σ(I)>	12.3	18.6	4.8
Completeness [%]	100.0	99.9	100
Redundancy	16.8	13.8	16.9
CC(1/2)	0.998	0.999	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.3 M sodium citrate tribasic dihydrate pH 5.6, 5% 2-propanol, 20% PEG 4000, 0.2% LMA