7F82

Structure of the bacterial cellulose synthase subunit Z in complex with cellooligosaccharides from Enterobacter sp. CJF-002

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-10
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	89.280, 92.860, 90.500
Unit cell angles	90.00, 98.41, 90.00

Refinement procedure

Resolution	42.510 - 1.300
R-factor	0.1698
Rwork	0.169
R-free	0.18660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7f81
RMSD bond length	0.014
RMSD bond angle	1.573
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.380
High resolution limit [Å]	1.300	1.300
Rmerge	0.030	0.567
Rmeas	0.033	0.669
Number of reflections	355775	57751
<I/σ(I)>	21.4	2.3
Completeness [%]	99.5	98.8
Redundancy	4.2	3.5
CC(1/2)	1.000	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M di-sodium tartrate, 20%(v/v) PEG3350