7F81

Structure of the bacterial cellulose synthase subunit Z from Enterobacter sp. CJF-002

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-18
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	89.267, 91.454, 89.931
Unit cell angles	90.00, 98.15, 90.00

Refinement procedure

Resolution	38.340 - 1.930
R-factor	0.164
Rwork	0.162
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qxf
RMSD bond length	0.009
RMSD bond angle	0.952
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	1.930	1.930
Rmerge	0.063	0.597
Rmeas	0.073	0.696
Number of reflections	107156	17297
<I/σ(I)>	18.26	2.57
Completeness [%]	99.7	99.3
Redundancy	3.8	3.8
CC(1/2)	0.999	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M di-sodium tartrate, 20%(v/v) PEG3350