7F7O
APC-Asef FP assay tracer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2020-11-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.234, 71.619, 83.873 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.460 - 2.800 |
R-factor | 0.2872 |
Rwork | 0.285 |
R-free | 0.32800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iz6 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.951 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.460 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.649 | |
Number of reflections | 8890 | 51835 |
<I/σ(I)> | 3.4 | |
Completeness [%] | 99.4 | |
Redundancy | 5.8 | |
CC(1/2) | 0.990 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M Ammonium sulfate, 0.1M Tris pH 8.0, 25% w/v PEG 3350 |