7F7H
SARS-CoV-2 S protein RBD in complex with A8-1 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.071 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.772, 75.660, 181.544 |
| Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
| Resolution | 36.280 - 3.190 |
| R-factor | 0.2087 |
| Rwork | 0.205 |
| R-free | 0.27530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vsb |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.304 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Number of reflections | 25319 | 2432 |
| <I/σ(I)> | 7.065 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.969 | 0.585 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris, PEG 6000, 100mM NaCl |
