7F7E

SARS-CoV-2 S protein RBD in complex with A5-10 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.071
Spacegroup name	H 3
Unit cell lengths	157.189, 157.189, 98.319
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.370 - 2.490
R-factor	0.2164
Rwork	0.214
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vsb
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.579
High resolution limit [Å]	2.490	2.490
Number of reflections	30468	2375
<I/σ(I)>	14.22
Completeness [%]	96.0
Redundancy	9.6
CC(1/2)	0.995	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris, PEG 6000, 100mM NaCl