7F74
Rv3094c in complex with FMN.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 289.15 |
| Detector technology | CCD |
| Collection date | 2019-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.058, 134.223, 109.411 |
| Unit cell angles | 90.00, 128.93, 90.00 |
Refinement procedure
| Resolution | 42.550 - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kcf |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.550 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 123413 | 12343 |
| <I/σ(I)> | 9.19 | |
| Completeness [%] | 94.5 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.995 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 289.15 | 0.2M sodium nitrate, 0.1M Bis Tris propane pH=8.5, 20% w/v PEG3350 |
