7F6Y

Complex Structure of antibody BD-503 and RBD-E484K of COVID-19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-06
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	C 2 2 21
Unit cell lengths	80.280, 149.071, 146.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 3.000
R-factor	0.2031
Rwork	0.198
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ejz
RMSD bond length	0.009
RMSD bond angle	1.053
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.200	0.760
Rmeas	0.220	0.820
Rpim	0.080	0.300
Number of reflections	33810	1762
<I/σ(I)>	8.36	2.12
Completeness [%]	99.5
Redundancy	6.8
CC(1/2)	0.990	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Magnesium chloride hexahydrate, 0.1M Sodium citrate, pH 5.0, 15% (w/v) PEG 4000