7F6W

Crystal structure of Saccharomyces cerevisiae lysyl-tRNA Synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	111.100, 111.100, 239.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.486 - 2.607
R-factor	0.1741
Rwork	0.172
R-free	0.20340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bju
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.650	48.650	2.690
High resolution limit [Å]	2.600	10.070	2.600
Rmerge	0.112	0.032	2.202
Rmeas	0.117	0.033	2.293
Rpim	0.033	0.010	0.634
Total number of observations	600863	9067	58456
Number of reflections	46870	930	4515
<I/σ(I)>	15.8	40.2	1.6
Completeness [%]	99.8	98.7	99.7
Redundancy	12.8	9.7	12.9
CC(1/2)	0.999	0.999	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Sodium HEPES, PEG 500 MME