7F69
Crystal structure of WIPI2b in complex with ATG16L1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97917 |
Spacegroup name | P 63 |
Unit cell lengths | 97.944, 97.944, 65.581 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.800 - 1.500 |
R-factor | 0.1828 |
Rwork | 0.182 |
R-free | 0.19670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ltg |
RMSD bond length | 0.012 |
RMSD bond angle | 1.147 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19rc4_4035) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.232 | 1.379 |
Number of reflections | 57102 | 2848 |
<I/σ(I)> | 9.06 | |
Completeness [%] | 98.4 | |
Redundancy | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 30% w/v Pentaerythritol ethoxylate (15/4 EO/OH), 100 mM MES (pH 6.5), 50 mM Magnesium chloride |