7F60
Crystal structure of auxiliary protein in complex with human nuclear protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.345, 87.531, 48.230 |
Unit cell angles | 90.00, 93.68, 90.00 |
Refinement procedure
Resolution | 48.240 - 2.850 |
R-factor | 0.2377 |
Rwork | 0.236 |
R-free | 0.27560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4owr |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.270 | 3.020 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.370 | 1.540 |
Number of reflections | 36291 | 5780 |
<I/σ(I)> | 3.47 | 0.8 |
Completeness [%] | 99.0 | 97.4 |
Redundancy | 3.52 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | 0.1m Bis-tris ph5.5,45%PEG4000 |