7F5Z
Crystal structure of the single-stranded dna-binding protein from Mycobacterium tuberculosis- Form III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-03-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 110.040, 110.040, 104.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.080 - 3.000 |
| R-factor | 0.25024 |
| Rwork | 0.248 |
| R-free | 0.28139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ue1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.365 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.080 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.183 | 0.883 |
| Number of reflections | 7920 | 1127 |
| <I/σ(I)> | 8.7 | 2.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 35% v/v 1,4-Dioxane |
