7F5Y
Crystal structure of the single-stranded dna-binding protein from Mycobacterium tuberculosis- Form III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-01-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 59.550, 59.550, 124.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.000 - 1.920 |
| R-factor | 0.19328 |
| Rwork | 0.191 |
| R-free | 0.22864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ue1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.457 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.020 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.088 | 0.744 |
| Number of reflections | 18944 | 2650 |
| <I/σ(I)> | 10.9 | 2.2 |
| Completeness [%] | 96.6 | 94.1 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 3.5M sodium formate, 100mM bis-tris propane pH 7.0 |
