7F5R

Crystal structure of SARS-CoV-2 Y453F-RBD bound to mink ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	P 21 21 2
Unit cell lengths	290.185, 130.185, 136.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.530 - 3.010
R-factor	0.1831
Rwork	0.181
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.530	3.120
High resolution limit [Å]	3.010	3.010
Rpim	0.078	0.447
Number of reflections	101471	9547
<I/σ(I)>	7.71
Completeness [%]	98.1
Redundancy	9.9
CC(1/2)	0.977	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	1.6M sodium/potassium phosphate