7F5M

Crystal structure of Sas5 YEATS domain in complex with H3K27bz peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 32 2 1
Unit cell lengths	126.578, 126.578, 48.708
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.400 - 2.400
R-factor	0.2294
Rwork	0.227
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d7e
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.080	0.031	0.899
Rmeas	0.087	0.034	0.971
Rpim	0.033	0.013	0.364
Total number of observations	118325
Number of reflections	17766	953	894
<I/σ(I)>	5.6
Completeness [%]	99.9	99.2	100
Redundancy	6.7	6.2	6.9
CC(1/2)		0.999	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	30% (w/v) PEG 4000, 0.2M Lithium sulfate, 0.1M Tris-Hcl